General

Cell Line

hPSCreg name DMBi008-A
Cite as:
DMBi008-A (RRID:CVCL_D0E7)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
QBRIi005-A
Donor diseases:
Psoriasis
QBRIi005-B
Donor diseases:
Psoriasis
QBRIi005-C
Donor diseases:
Psoriasis
DMBi007-A
Donor diseases:
Psoriasis
QBRIi006-A
Donor diseases:
psoriasis
QBRIi006-B
Donor diseases:
psoriasis
QBRIi006-C
Donor diseases:
psoriasis
UQACi007-A
(iPSC-EBS29)
Donor's gene variants:
Keratin 14
Donor diseases:
epidermolysis bullosa simplex
UQACi005-A
(iPSC-EBS10)
Donor's gene variants:
Keratin 14
Donor diseases:
epidermolysis bullosa simplex
UQACi006-A
(iPSC-EBS26)
Donor's gene variants:
keratin 5
Donor diseases:
epidermolysis bullosa simplex
Last update 6th November 2023
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Provider

Generator Department of Medical Biotechnology (DMB)

External Databases

BioSamples SAMEA114216501
Cellosaurus CVCL_D0E7
Wikidata Q123031173

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Psoriasis
  • psoriasis

External Databases (Donor)

BioSamples SAMEA114216498

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Department of Dermatology, Venereology and Allergology, Wroclaw Medical University, Poland
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Wroclaw Medical University Bioethical Committee
Approval number 110/2020
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)