Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells


Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29. Our protocol offers a non-invasive and efficient approach for iPSC generation from patients with genetic or acquired disorders. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Boonkaew B, Thummavichit W, Netsrithong R, Vatanashevanopakorn C, Pattanapanyasat K, Wattanapanitch M
Journal Stem cell research
Publication Date 2018 Jul;30:34-37
PubMed 29778975
DOI 10.1016/j.scr.2018.05.002

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