m55Alz-9L

General#

Cell Line

hPSCreg Name ICGi008-A
Alternative name(s)
m55Alz-9L
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 26th December 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator The Federal Research Center Institute of Cytology and Genetics The Siberian Branch of the Russian Academy of Sciences (ICG)
Owner The Federal Research Center Institute of Cytology and Genetics The Siberian Branch of the Russian Academy of Sciences (ICG)
Distributors
Derivation country Russia

External Databases

BioSamples SAMEA5341576
Cellosaurus CVCL_UJ81

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Alzheimer's disease
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Disease, Alzheimer
  • Dementia in Alzheimer's disease, unspecified (disorder)
  • Presenile Alzheimer Dementia
  • ALZHEIMERS DIS
  • Alzheimers
  • DAT - Dementia Alzheimer's type
  • Dementia in Alzheimer's disease
  • Alzheimer's disease, NOS
  • Alzheimer Dementia, Presenile
  • Dementia, Alzheimer Type
  • Alzheimer Dementia
  • Alzheimer's Dementia
  • Alzheimer's
  • Dementia, Presenile
  • [X]Dementia in Alzheimer's disease (disorder)
  • ALZHEIMER DIS
  • AD
  • [X]Dementia in Alzheimer's disease
  • AD - Alzheimer's disease
  • Disease, Alzheimer's
  • Alzheimer Disease
  • Dementia, Presenile Alzheimer
  • Alzheimer Type Dementia
  • Dementia in Alzheimer's disease (disorder)
  • Alzheimer's disease (disorder)
  • Alzheimers Dementia
  • Dementia of the Alzheimer's type
  • sporadic Alzheimer's disease
  • Alzheimers disease
show more synonyms
Disease associated phenotypes
  • Alzheimer’s disease

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5341577

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name m55Alz
Derived from same source line (potentially other lot and donor, see below):
Source cell type
fibroblast of dermis
Source cell origin
skin of back
A zone of skin that is part of a back [Automatically generated definition].
Synonyms
  • back zone of skin
  • zone of skin of back
  • back skin
  • skin, dorsal region
show more synonyms
Source cell line lot number
None
Age of donor (at collection) 55-59
Collected in 2013
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones The selection of colonies was carried out according to morphological criteria. We selected flat monolayer colonies with tightly packed cells with high nuclear/cytoplasmic ratio. Embryonic stem cell-like clones were picked by a glass microcapillary.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells mouse emryonic fibroblasts
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout DMEM (Life Technologies)
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
NEAA 0.1 mM
GlutaMAX 2 mM
2-mercaptoethanol 0.1 mM
rhFGF basic 10 ng/ml
Penicillin 50 U/ml
Streptomycin 50 µg/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Alkaline Phosphatase
Yes
Morphology pictures
m55Alz-9L-1.tif
Morphology of ICGi008-A iPSC, the scale bar - 500 mcm.
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CK18
Yes
GATA6
Yes
Morphology
Spont dif m55Alz-9L GATA6_g KRT18_r.tif
Immunofluorescent analysis for endoderm markers GATA6 (green signal) and Cytokeratin18 (red signal) in ICGi008-A IPSC, DAPI (blue signal), the scale bar - 100 mcm.
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Morphology
Immunofluorescent analysis for mesoderm marker a-SMA (red signal) in ICGi008-A IPSC, DAPI (blue signal), the scale bar - 100 mcm.
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Neurofilament 200
Yes
Morphology
Immunofluorescent analysis for marker ectoderm Neurofilament 200 (green signal) in ICGi008-A iPSC, DAPI (blue signal), the scale bar - 100 mcm.

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyotyping and G-banding show ICGi008-A iPSC have a normal 46, XX karyotype.
Karyotyping method: G-Banding

Other Genotyping (Cell Line)