RC10, RC-10

General

Cell Line

hPSCreg name RCe014-A
Cite as:
RCe014-A (RRID:CVCL_C781)
Alternative name(s)
RC10, RC-10
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 28th November 2017
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Provider

Generator Roslin Cells (RC)
Owner Derivation of Clinical Grade hES Cells
Distributors
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_C781
Wikidata Q54949370

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? HFEA

hESC Derivation

Date of derivation 2009-10-13
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Spontaneous Hatching
Cell isolation None
Cell seeding Embryo

Culture Conditions

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)