Generation of induced pluripotent stem cells (IBMSi011-A) from a patient with Parkinson's disease carrying LRRK2 p.I1371V mutation


Leucine rich repeat kinase 2 (LRRK2) is the causative gene for autosomal-dominant familial forms of Parkinson's disease (PD). Here, we generated induced pluripotent stem cells (iPSCs) from the peripheral blood mononuclear cells of a female patient with LRRK2 c.4111A > G (p.I1371V) mutation by using the Sendai-virus delivery system. The resulting iPSCs had a normal karyotype. The iPSCs also showed pluripotency confirmed by immunofluorescent staining and differentiated into the three germ layers in vivo. This cellular model will provide a platform for studying the role of LRRK2 in the disease process. Copyright © 2019. Published by Elsevier B.V.

Authors Lin HI, Cheng YC, Ko HW, Wen CH, Lu HE, Huang CY, Hsieh PCH, Lin CH
Journal Stem cell research
Publication Date 2019 Apr 22;37:101447
PubMed 31029016
DOI 10.1016/j.scr.2019.101447

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