Generation of induced pluripotent stem cells (IBMSi011-A) from a patient with Parkinson's disease carrying LRRK2 p.I1371V mutation
Summary
Leucine rich repeat kinase 2 (LRRK2) is the causative gene for autosomal-dominant familial forms of Parkinson's disease (PD). Here, we generated induced pluripotent stem cells (iPSCs) from the peripheral blood mononuclear cells of a female patient with LRRK2 c.4111A > G (p.I1371V) mutation by using the Sendai-virus delivery system. The resulting iPSCs had a normal karyotype. The iPSCs also showed pluripotency confirmed by immunofluorescent staining and differentiated into the three germ layers in vivo. This cellular model will provide a platform for studying the role of LRRK2 in the disease process. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Lin HI, Cheng YC, Ko HW, Wen CH, Lu HE, Huang CY, Hsieh PCH, Lin CH |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2019 May;37:101447 |
| PubMed | 31029016 |
| DOI | 10.1016/j.scr.2019.101447 |