Generation of induced pluripotent stem cells (IBMSi011-A) from a patient with Parkinson's disease carrying LRRK2 p.I1371V mutation


Leucine rich repeat kinase 2 (LRRK2) is the causative gene for autosomal-dominant familial forms of Parkinson's disease (PD). Here, we generated induced pluripotent stem cells (iPSCs) from the peripheral blood mononuclear cells of a female patient with LRRK2 c.4111A > G (p.I1371V) mutation by using the Sendai-virus delivery system. The resulting iPSCs had a normal karyotype. The iPSCs also showed pluripotency confirmed by immunofluorescent staining and differentiated into the three germ layers in vivo. This cellular model will provide a platform for studying the role of LRRK2 in the disease process. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Lin HI, Cheng YC, Ko HW, Wen CH, Lu HE, Huang CY, Hsieh PCH, Lin CH
Journal Stem cell research
Publication Date 2019 May;37:101447
PubMed 31029016
DOI 10.1016/j.scr.2019.101447

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