HS916

General

Cell Line

hPSCreg name KIe051-A
Cite as:
KIe051-A (RRID:CVCL_HF72)
Alternative name(s)
HS916
Cell line type Human embryonic stem cell (hESC)
Similar lines
RGIe049-A
(SI-137)
Donor diseases:
neurofibromatosis
RGIe050-A
(SI-138)
Donor diseases:
neurofibromatosis
RGIe134-A
(SI-235)
Donor diseases:
neurofibromatosis
RGIe051-A
(SI-139)
Donor diseases:
neurofibromatosis
RGIe052-A
(SI-140)
Donor diseases:
neurofibromatosis
Last update 24th April 2020
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Provider

Generator Karolinska Institutet (KI)
Owner Stem Cell Laboratory
Distributors
Derivation country Sweden

External Databases

BioSamples SAMEA6878150
Cellosaurus CVCL_HF72
Wikidata Q54896342

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Neurofibromatosis
  • Neurofibromatosis Syndrome
  • Neurofibromatosis, NOS

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA6878152

Ethics

Was the embryo established purely for research purposes? Yes
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink ganesh.acharya@ki.se
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Regional Ethics Committee, Stockholm, Sweden
Approval number 454/02
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Regional Ethics Committee, Stockholm, Sweden
Approval number 454/02

hESC Derivation

Date of derivation 2011-12-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
Yes
ZP removal technique Mechanical
Cell isolation Mechanical
Cell seeding Isolated ICM
Derived under xeno-free conditions?
Yes
Derivation under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Laminin
Feeder cells
No
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: NutriStem hESC XF
Main protein source: Human Serum
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
smooth muscle actin
Unknown
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
MAP2
Unknown

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)