Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene


The leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription factors. The generated iPSC line exhibits expression of common pluripotency markers, differentiates into the three germ layers and has a normal karyotype. The iPSC line can be used to explore the association between LRRK2 mutation and PD. Copyright © 2017. Published by Elsevier B.V.

Authors Marote A, Pomeshchik Y, Collin A, Goldwurm S, Lamas NJ, Pinto L, Salgado AJ, Roybon L
Journal Stem cell research
Publication Date 2018 Apr;28:44-47
PubMed 29414418
DOI 10.1016/j.scr.2018.01.022

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