Generation and characterization of two iPSC lines from human adipose tissue-derived stem cells of healthy donors


Here we have generated two induced pluripotent stem cell (iPSC) lines, hASC-iPSC-1A and hASC-iPSC-2A, by reprogramming human adipose tissue derived stem cells of 28 and 23 years old healthy donors. Reprogramming was achieved using nonintegrative Sendai viral vector system containing the reprogramming factors Klf4, Oct3/4, Sox2, c-Myc. Though the karyotypes of these cells were normal (46, XX) and (46, XY), their pluripotency potentials were confirmed by the expression of factors of pluripotency markers in vitro and teratoma formation in vivo. These iPSCs differentiated cells can serve as control for disease modeling and drug screening. Copyright © 2020. Published by Elsevier B.V.

Authors Zhou XB, Li H, Li F, Song XK, Liu T, Ma T, Guo HY, Wu N, Li J
Journal Stem cell research
Publication Date 2020 Oct;48:101973
PubMed 32896746
DOI 10.1016/j.scr.2020.101973

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