UPSFRe002-A

CL02

General#

Cell Line

hPSCreg Name UPSFRe002-A
Alternative name(s)
CL02
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Université Paris-Sud 11 (UPSFR)
Contact:
ESTeam Paris Sud
Derivation country France

External Databases

Cellosaurus CVCL_C797

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
monogenic disease
The donor is affected.

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2008-12-09
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
Expansion status 1
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Mechanical
Cell isolation Laser
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells Mouse Embryonic Fibroblast
Cellfinder Ont Id: EFO_0004040
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
2-mercaptoetanol
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
CD9
Yes
GABRB3
Yes
LEFTB
Yes
LIN28
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TDGF1
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
HESCA-1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX.arr(1-22,X)x2

Other Genotyping (Cell Line)