General

Cell Line
hPSCreg name	RCe009-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	RCe009-A (RRID:CVCL_C787)
Alternative name(s)	RC-5, RC5
Cell line type	Human embryonic stem cell (hESC)
Similar lines	SDUe007-A (KMEB2) Donor diseases: monogenic disease RGIe115-A (SI-213) Donor diseases: monogenic disease KCLe004-A (KCL-005_HD1) Donor diseases: monogenic disease UPSFRe002-A (CL02) Donor diseases: monogenic disease RCe010-A (RC-6, RC6) Donor diseases: monogenic disease RGIe145-A (SI-246) Donor diseases: monogenic disease
Last update	28th November 2017
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Provider
Generator	Roslin Cells (RC) Contact: Derivation of Clinical Grade hES Cells
Owner	Derivation of Clinical Grade hES Cells
Distributors	Derivation of Clinical Grade hES Cells National Institute for Biological Standards and Control - UK Stem Cell Bank (USCB)
Derivation country	United Kingdom
External Databases
Cellosaurus	CVCL_C787
Wikidata	Q54949404
General Information
Publications	De Sousa PA et al. Derivation of the human embryonic stem cell line RCe009-A (RC-5). Stem cell research. 2016 Mar;16(2):418-22. De Sousa PA et al. Development and production of good manufacturing practice grade human embryonic stem cell lines as source material for clinical application. Stem cell research. 2016 Sep;17(2):379-390.
* Is the cell line readily obtainable for third parties?	Yes

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Monogenic disease The donor is affected.

Ethics

Was the embryo established purely for research purposes?	No
Have both parents consented to the use of the embryo for ESC derivation?	Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Alternatives to consent are available?	Yes
Alternatives to consent
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent expressly prevent the derivation of pluripotent stem cells?	No
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	HFEA
Approval number	R0136

hESC Derivation

Date of derivation	2007-10-24
Embryo stage	Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?	Yes
PGD Embryo?	No
Cell isolation	None
Cell seeding	Embryo

Culture Conditions

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
NANOG	Yes
POU5F1 (OCT-4)	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro spontaneous differentiation

Microbiology / Virus Screening
HIV 1	Negative
HIV 2	Negative
Hepatitis B	Negative
Hepatitis C	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XX Passage number: 17
Other Genotyping (Cell Line)