Derivation of the human embryonic stem cell line RCe009-A (RC-5)
Summary
The human embryonic stem cell line RCe009-A (RC-5) was derived from a frozen and thawed Day 2 embryo voluntarily donated as unsuitable and surplus to requirement for fertility treatment following informed consent under licence from the UK Human Fertilisation and Embryology Authority. RCe009-A carries the common DF508 mutation on the cystic fibrosis trans-membrane regulator gene associated with the disease cystic fibrosis. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data are available. Copyright © 2016 University of Texas at Austin Dell Medical School. Published by Elsevier B.V. All rights reserved.
| Authors | De Sousa PA, Tye B, Bruce K, Dand P, Russell G, Collins DM, Gardner J, Downie JM, Bateman M, Courtney A |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2016 Mar;16(2):418-22 |
| PubMed | 27346004 |
| DOI | 10.1016/j.scr.2016.02.030 |