Derivation of the human embryonic stem cell line RCe009-A (RC-5)


The human embryonic stem cell line RCe009-A (RC-5) was derived from a frozen and thawed Day 2 embryo voluntarily donated as unsuitable and surplus to requirement for fertility treatment following informed consent under licence from the UK Human Fertilisation and Embryology Authority. RCe009-A carries the common DF508 mutation on the cystic fibrosis trans-membrane regulator gene associated with the disease cystic fibrosis. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data are available. Copyright © 2016 University of Texas at Austin Dell Medical School. Published by Elsevier B.V. All rights reserved.

Authors De Sousa PA, Tye B, Bruce K, Dand P, Russell G, Collins DM, Gardner J, Downie JM, Bateman M, Courtney A
Journal Stem cell research
Publication Date 2016 Mar;16(2):418-22
PubMed 27346004
DOI 10.1016/j.scr.2016.02.030

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