General

Cell Line
hPSCreg name	RGIe145-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	RGIe145-A (RRID:CVCL_B665)
Alternative name(s)	SI-246
Cell line type	Human embryonic stem cell (hESC)
Similar lines	KCLe004-A (KCL-005_HD1) Donor diseases: monogenic disease RCe009-A (RC-5, RC5) Donor diseases: monogenic disease RCe010-A (RC-6, RC6) Donor diseases: monogenic disease SDUe007-A (KMEB2) Donor diseases: monogenic disease RGIe115-A (SI-213) Donor diseases: monogenic disease UPSFRe002-A (CL02) Donor diseases: monogenic disease
Last update	4th September 2022
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Provider
Generator	Reproductive Genetics Institute (RGI) Contact: Stem Cell Research Unit
Derivation country	United States
External Databases
Cellosaurus	CVCL_B665
Wikidata	Q54950031
General Information
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Monogenic disease The donor is affected.
Disease associated phenotypes	monogenetic disease

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Supernumerary embryos from IVF treatment?	Yes
PGD Embryo?	Yes

Culture Conditions

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
POU5F1 (OCT-4)	Yes
SSEA-3	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes
TRA 2-39	Yes

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes Passage number: 7
Other Genotyping (Cell Line)