Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype


A 62-year old sporadic Alzheimer's disease (sAD) male patient with APOE3 (ε3/ε3) genotype donated his peripheral blood mononuclear cells (PBMCs). The induced pluripotent stem cell (iPSC) line was established by episomal vector system with the four factors OCT4, SOX2, C-MYC and KLF4. EB differentiation in vitro showed that iPSC line had a potential to differentiate into three germ layers. More than 90% cells expressed NANOG, OCT4 and SSEA4 detected by flow cytometry. In addition, the iPSC line was karyotypically normal. The iPSC line may provide new valuable tools for studying pathogenesis of sAD and screening candidate drugs for the disease. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhang L, Xu M, Liu G, Wu R, Meng S, Xiahou K, Jiang N, Zhang Y, Zhou W
Journal Stem cell research
Publication Date 2019 Dec;41:101589
PubMed 31689594
DOI 10.1016/j.scr.2019.101589

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