Generation and cardiac subtype-specific differentiation of PITX2-deficient human iPS cell lines for exploring familial atrial fibrillation


Loss-of-function mutations in the PITX2 transcription factor gene have been shown to cause familial atrial fibrillation (AF). To potentially model aspects of AF and unravel PITX2-regulated downstream genes for drug target discovery, we here report the generation of integration-free PITX2-deficient hiPS cell lines. We also show that both PITX2 knockout hiPS cells and isogenic wild-type controls can selectively be differentiated into human atrial cardiomyocytes, to potentially uncover differentially expressed gene sets between these groups. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Marczenke M, Fell J, Piccini I, Röpke A, Seebohm G, Greber B
Journal Stem cell research
Publication Date 2017 May;21:26-28
PubMed 28677534
DOI 10.1016/j.scr.2017.03.015

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