B-mC-KI-SE-3
SNUe003-A-2
General
Cell Line |
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| hPSCreg Name | SNUe003-A-2 |
| Alternative name(s) |
B-mC-KI-SE-3
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | |
| Last update | 14th April 2021 |
| User feedback | |
Provider |
|
| Generator |
Institute of Reproductive Medicine and Population, Medical Research Cener, (IRMP)
Contact:
Seoul National University (SNU) |
| Distributors | |
| Derivation country | South Korea |
External Databases |
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| BioSamples | SAMEA7513374 |
| CLO | CLO_0103109 |
| Cellosaurus | CVCL_A5MW |
| Wikidata | Q107116949 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Korean |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA7513375 |
Ethics
Also have a look at the ethics information for the parental line
SNUe003-A
.
| Is there an MTA available for the cell line? | Yes |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
|
The source cell information can be found in the parental cell line
SNUe003-A.
|
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
|
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Morphology pictures
REX1, OCT4 and TERT were detected by RT-PCR.
Method documentation
Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
In vitro spontaneous differentiation
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
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