SNUe003-A-2

B-mC-KI-SE-3

General#

Cell Line

hPSCreg Name SNUe003-A-2
Alternative name(s)
B-mC-KI-SE-3
Cell line type Human embryonic stem cell (hESC)
Last update 13th October 2020
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Provider

Generator Institute of Reproductive Medicine and Population, Medical Research Cener, (IRMP)
Distributors
Derivation country South Korea

External Databases

BioSamples SAMEA7513374

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Korean

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7513375

Ethics#

Also have a look at the ethics information for the parental line SNUe003-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation#

Date of derivation 2019-09-10
Supernumerary embryos from IVF treatment?
No
Other hESC source: engineered brachyury gene in SNUhES3
PGD Embryo?
No
Derived under xeno-free conditions?
Yes
Derivation under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
REX1, OCT4 and TERT were detected by RT-PCR.
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
HNF3B
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Enolase
Yes
Brachyury
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
nestin
Yes
vimentin
Yes
MAP1
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 46
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
Brachyury (target)
Gene knock-in
6q27
6q27
CRISPR-associated (CRISPR/Cas) System