SNUe003-A-1

General#

Cell Line

hPSCreg Name SNUe003-A-1
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator Seoul National University (SNU)
Derivation country South Korea

External Databases

CLO CLO_0102884

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Korean

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7513375

Ethics#

Also have a look at the ethics information for the parental line SNUe003-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
ZP removal technique Enzymatic
Cell isolation Mechanical

Culture Conditions#

Feeder cells STO cell line
Cellfinder Ont Id: CLO_0009149
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
Alkaline Phosphatase
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XY,+12
Passage number: 95

Other Genotyping (Cell Line)

Genetic Modification#