SNUe003-A-1
General
Cell Line |
|
| hPSCreg name | SNUe003-A-1 |
| Cite as: | SNUe003-A-1 (RRID:CVCL_A5MV) |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator |
Seoul National University (SNU)
Contact:
Stem Cell Biology |
| Derivation country | South Korea |
External Databases |
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| Cellosaurus | CVCL_A5MV |
| Wikidata | Q107116948 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Korean |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA7513375 |
Ethics
Also have a look at the ethics information for the parental line
SNUe003-A
.
| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
|
The source cell information can be found in the parental cell line
SNUe003-A.
|
Culture Conditions
| Feeder cells |
STO cell line Cellfinder Ont Id: CLO_0009149 |
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| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| Alkaline Phosphatase |
Yes |
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| SSEA-1 |
No |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
47XY,+12
Passage number: 95
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Other Genotyping (Cell Line) |
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