SNUe003-A-1

General

Cell Line

hPSCreg name SNUe003-A-1
Cite as:
SNUe003-A-1 (RRID:CVCL_A5MV)
Cell line type Human embryonic stem cell (hESC)
Similar lines
SNUe003-A
(SNUhES3)
SNUe003-A-2
(B-mC-KI-SE-3)
SNUe003-A-4
(T-mC-KI-SE-3)
SNUe003-A-3
(A-eG-KI-SE-3)
Last update 22nd May 2019
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Provider

Generator Seoul National University (SNU)
Derivation country South Korea

External Databases

Cellosaurus CVCL_A5MV
Wikidata Q107116948

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Korean

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7513375

Ethics

Also have a look at the ethics information for the parental line SNUe003-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

The source cell information can be found in the parental cell line SNUe003-A.

Culture Conditions

Feeder cells STO cell line
Cellfinder Ont Id: CLO_0009149
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
Alkaline Phosphatase
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XY,+12
Passage number: 95

Other Genotyping (Cell Line)

Genetic Modification