T-mC-KI-SE-3
SNUe003-A-4
General
Cell Line |
|
| hPSCreg name | SNUe003-A-4 |
| Cite as: | SNUe003-A-4 (RRID:CVCL_D0QP) |
| Alternative name(s) |
T-mC-KI-SE-3
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | |
| Last update | 9th March 2023 |
| User feedback | |
Provider |
|
| Generator | Institute of Reproductive Medicine and Population, Medical Research Cener, (IRMP) |
| Owner | Institute of Reproductive Medicine and Population, Medical Research Cener, (IRMP) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA12504963 |
| Cellosaurus | CVCL_D0QP |
| Wikidata | Q123033546 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Korean |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA7513375 |
Ethics
Also have a look at the ethics information for the parental line
SNUe003-A
.
| Is there an MTA available for the cell line? | Yes |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
|
The source cell information can be found in the parental cell line
SNUe003-A.
|
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
|
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
Morphology pictures
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
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| Genetic modifications not related to a disease |
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