SNUe003-A-4

T-mC-KI-SE-3

General#

Cell Line

hPSCreg Name
SNUe003-A-4
Alternative name(s)
T-mC-KI-SE-3
Cell line type Human embryonic stem cell (hESC)
Last update 21st January 2022
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Provider

Generator Institute of Reproductive Medicine and Population, Medical Research Cener, (IRMP)
Owner Institute of Reproductive Medicine and Population, Medical Research Cener, (IRMP)
Distributors

External Databases

BioSamples SAMEA12504963
CLO CLO_0103741

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Korean

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7513375

Ethics#

Also have a look at the ethics information for the parental line SNUe003-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation#

The source cell information can be found in the parental cell line SNUe003-A.

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
sox17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Brachyury
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
otx
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 49
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
(unknown disease)
Genetic modifications
TUBB3 (target)
Gene knock-in
16q24.3
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease
TUBB3(Tuj1) (target)
Gene knock-in
16q24.3
CRISPR-associated (CRISPR/Cas) System