SNUe003-A-3

A-eG-KI-SE-3

General#

Cell Line

hPSCreg Name
SNUe003-A-3
Alternative name(s)
A-eG-KI-SE-3
Cell line type Human embryonic stem cell (hESC)
Last update 14th July 2021
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Provider

Generator Institute of Reproductive Medicine and Population, Medical Research Cener, (IRMP)
Owner Seoul National University (SNU)
Distributors
Derivation country South Korea

External Databases

BioSamples SAMEA9403864
CLO CLO_0103484

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Korean

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7513375

Ethics#

Also have a look at the ethics information for the parental line SNUe003-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation#

Date of derivation 2019-09-08
Supernumerary embryos from IVF treatment?
No
Other hESC source: a subclone of SNUe003
PGD Embryo?
No
Derived under xeno-free conditions?
No
Derivation under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Alkaline Phosphatase
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
sox17
Yes
Cardiac Muscle Cell
Ont Id: CL_0000746
Marker Expressed
Brachyury
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
otx
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 46
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
(unknown disease)
Genetic modifications
alpha-MHC (target)
Gene knock-in
14q11.2
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease
alpha-MHC (target)
Gene knock-in
14q11.2