Generation of αMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting


The cardiac muscle-specific protein, α-myosin heavy chain (αMHC), is a major component of cardiac muscle filaments involved in cardiac muscle contraction. Here, we established an αMHC-enhanced fluorescent protein (EGFP) knock-in human pluripotent stem cell (hPSC) line by linking the EGFP gene to the C-terminal region of αMHC via a 2A non-joining peptide using CRISPR/Cas9 nuclease. The EGFP reporter precisely reflected the endogenous level of αMHC upon the induction of cardiac differentiation. This reporter cell line will be a valuable platform for cardiotoxicity tests, drug screening, and investigating the pathological mechanisms of cardiomyocytes. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Lee HM, Kim AH, Hwang S, Jung J, Seol H, Sung JJ, Jeong SM, Choi YM, Jun JK, Kim HS, Jang J
Journal Stem cell research
Publication Date 2022 May;61:102779
PubMed 35427904
DOI 10.1016/j.scr.2022.102779

Research Projects

Cell Lines