Generation of αMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting
Summary
The cardiac muscle-specific protein, α-myosin heavy chain (αMHC), is a major component of cardiac muscle filaments involved in cardiac muscle contraction. Here, we established an αMHC-enhanced fluorescent protein (EGFP) knock-in human pluripotent stem cell (hPSC) line by linking the EGFP gene to the C-terminal region of αMHC via a 2A non-joining peptide using CRISPR/Cas9 nuclease. The EGFP reporter precisely reflected the endogenous level of αMHC upon the induction of cardiac differentiation. This reporter cell line will be a valuable platform for cardiotoxicity tests, drug screening, and investigating the pathological mechanisms of cardiomyocytes. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Lee HM, Kim AH, Hwang S, Jung J, Seol H, Sung JJ, Jeong SM, Choi YM, Jun JK, Kim HS, Jang J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 May;61:102779 |
| PubMed | 35427904 |
| DOI | 10.1016/j.scr.2022.102779 |