AG27

General#

Cell Line

hPSCreg Name UIOi001-A
Alternative name(s)
AG27
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 3rd July 2019
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Provider

Generator University of Oslo (UIO)
Owner University of Oslo (UIO)
Distributors
Derivation country Norway

External Databases

BioSamples SAMEA5928374

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history No
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XX
Karyotyping method: Chromosome analysis

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array
https://www.ncbi.nlm.nih.gov/SNP/snp_ind.cgi?ind_id=21457
4% of the cells examined showing random chromosome loss/gain and 2% showing random chromosomal aberrations. Donor is the cousin of AG12427.

External Databases (Donor)

BioSamples SAMEA5928372

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: https://www.coriell.org/1/NIA
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink https://www.coriell.org/1/NIA
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? Yes
Alternatives to consent This cell line was obtained from the National Institute on Aging (NIA) Aging Cell Repository. The NIA is an NIH institute.
Alternative consent approval number https://www.coriell.org/1/NIA
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
an academic institution? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell line name AG05836
Source cell type
Fibroblast
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
Source cell origin
upper arm skin
A zone of skin that is part of a forelimb stylopod [Automatically generated definition].
Synonyms
  • skin of upper arm
  • skin of arm stylopod
  • arm stylopod skin
Source cell line vendor Coriell
Passage number reprogrammed 27

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
Unknown

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
Morphology pictures
ReprogrammingPics.pdf
Morphology of fibroblasts (brightfield) after reprogramming
Differentiation Potency
Hepatocyte
Ont Id: CL_0000182
In vitro directed differentiation
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Marker Expressed
NR2F2
Yes
HEY2
No
MYL2
Yes
NKX2-5
No
TNNT2
Yes
Protocol or reference
Cardiomyocyte_qPCR.pdf
gene expression for directed differentiation of cardiomyocytes
Neuron
Ont Id: CL_0000540
In vitro directed differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX

Other Genotyping (Cell Line)