AG27
UIOi001-A
General
Cell Line |
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| hPSCreg name | UIOi001-A |
| Cite as: | UIOi001-A (RRID:CVCL_YC60) |
| Alternative name(s) |
AG27
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 3rd July 2019 |
| User feedback | |
Provider |
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| Generator | University of Oslo (UIO) |
| Owner | University of Oslo (UIO) |
| Distributors | |
| Derivation country | Norway |
External Databases |
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| BioSamples | SAMEA5928374 |
| Cellosaurus | CVCL_YC60 |
| Wikidata | Q98133887 |
General Information |
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| Publications |
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| Projects | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
| Family history | No |
| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46,XX
Karyotyping method:
Chromosome analysis
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
https://www.ncbi.nlm.nih.gov/SNP/snp_ind.cgi?ind_id=21457 4% of the cells examined showing random chromosome loss/gain and 2% showing random chromosomal aberrations. Donor is the cousin of AG12427. |
External Databases (Donor) |
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| BioSamples | SAMEA5928372 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | No |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | https://www.coriell.org/1/NIA |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Contact information / weblink | https://www.coriell.org/1/NIA |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | This cell line was obtained from the National Institute on Aging (NIA) Aging Cell Repository. The NIA is an NIH institute. |
| Alternative consent approval number | https://www.coriell.org/1/NIA |
| Has the donor agreed to be re-contacted? | Unknown |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| How may genetic information associated with the cell line be accessed? | Open Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
hIPSC Derivation
General |
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| Source cell line name | AG05836 |
| Source cell type |
Synonyms
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| Source cell origin |
A zone of skin that is part of a forelimb stylopod [Automatically generated definition].
Synonyms
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| Source cell line vendor | Coriell |
| Passage number reprogrammed | 27 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
Morphology pictures
ReprogrammingPics.pdf
Morphology of fibroblasts (brightfield) after reprogramming
Differentiation Potency
In vitro directed differentiation
Protocol or reference
Cardiomyocyte_qPCR.pdf
gene expression for directed differentiation of cardiomyocytes
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XX
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Other Genotyping (Cell Line) |
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