Generation of induced pluripotent stem cells from an HLA-B27 positive ankylosing spondylitis patient with syndesmophyte formation
Summary
Human leukocyte antigen (HLA)-B27 is the genetic marker for ankylosing spondylitis (AS). Here, we generated induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells of a male AS patient carrying HLA-B27 with syndesmophyte formation by using the Sendai-virus delivery system. The resulting iPSCs had a normal karyotype, expressed pluripotent markers, and could differentiate into three germ layers. This cellular model will provide a platform for studying pathological mechanisms of new bone formation in HLA-B27 positive AS patients. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Tsai WH, Lin YC, Lan SH, Lin YH, Chen CH, Hsieh SC, Liu CH |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Oct;80:103508 |
| PubMed | 39079398 |
| DOI | 10.1016/j.scr.2024.103508 |