General

iPSC Line
hPSCreg name	BRCi014-A-3
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	BRCi014-A-3 (RRID:CVCL_E3Y8)
Alternative name(s)	MBP-TEZ
iPSC line type	Human induced pluripotent stem cell (hiPSC)
Similar iPSC lines	BRCi014-A (1383D6, HPS1006)
Last update	27th April 2024
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Provider
Generator	RIKEN BioResource Research Center (BRC)
External iPSC Databases
BioSamples	SAMEA115499868
Cellosaurus	CVCL_E3Y8
General iPSC Information
Publications	Takagi D et al. Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization. Stem cell research. 2024 Sep;79:103493.
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	BRCi014-A

iPSC Genetic Modification

Disease/phenotype related modifications	Oligodendrocyte Synonyms Oligodendrocyte Oligodendrocytes Oligodendroglia Oligodendroglia Cell oligodendrocyte show more synonyms show less synonyms Genetic modifications MBP (target) Type of iPSC Modification Gene knock-in Chromosome location 18q23
Genetic modifications not related to a disease	MBP (target) Type of iPSC Modification Gene knock-in Chromosome location 18q23

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
External Databases (Donor)
BioSamples	SAMEA115527588

Ethics

Also have a look at the ethics information for the parental line BRCi014-A .

For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

iPSC Derivation

General
The source cell information can be found in the parental cell line BRCi014-A.
Reprogramming method
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

iPSC Culture Conditions

iPSC Characterisation

Analysis of undifferentiated iPSCs

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
POU5F1 (OCT-4)	Yes	–
NANOG	Yes	–

Morphology

Morphology pictures

MBP-TEZ_phasecontrast_scale200um.tif

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Marker	Expressed
AFP	Yes
FOXA2	Yes

Morphology

MBP-TEZ_AFP_x20_scale200um.tif

MBP-TEZ_FOXA2_x20_scale200um.tif

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

Marker	Expressed
HAND1	Yes
SMA	Yes

Morphology

MBP-TEZ_HAND1_x20_scale200um.tif

MBP-TEZ_SMA_x20_scale200um.tif

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro spontaneous differentiation

Marker	Expressed
TUJ1	Yes
PAX6	Yes

Morphology

MBP-TEZ_PAX6_x20_scale200um.tif

MBP-TEZ_TUJ1_x20_scale200um.tif

Genotyping

Karyotyping (iPSC Line)

Other Genotyping (iPSC Line)