Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing
Summary
We used gene editing to introduce DNA sequences encoding the tdTomato fluorescent protein into the α -skeletal actin 1 (ACTA1) locus to develop an ACTA1-tdTomato induced pluripotent stem cell reporter line for monitoring differentiation of skeletal muscle. This cell line will be used to better understand skeletal muscle maturation and development in vitro as well as provide a useful tool for drug screening and the evaluation of novel therapeutics for the treatment of skeletal muscle disease. Copyright © 2024. Published by Elsevier B.V.
| Authors | Houweling PJ, Crossman V, Tiong CF, Coles CA, Taylor RL, Clayton JS, Graham A, Vlahos K, Howden SE, North KN |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Mar;75:103313 |
| PubMed | 38277710 |
| DOI | 10.1016/j.scr.2024.103313 |