MCRIi001-A-SOX9tdTom, PB001-SOX9tdTom
MCRIi001-A-2
General
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA4966491 |
Ethics
Also have a look at the ethics information for the parental line
MCRIi001-A
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| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | Only available for agreed collaborative projects and with an MTA in place |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
MCRIi001-A.
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Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Non coated | |||||||||||||||
| Feeder cells |
MEF |
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| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 95 % | |||||||||||||||
| CO2 Concentration | 5 % | |||||||||||||||
| Medium |
Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Molcular karyotype arr(1-22)x2(XY)x1
Passage number: 2
Karyotyping method:
Molecular karyotyping by SNP array
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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