Generation of a SOX9-tdTomato reporter human iPSC line, MCRIi001-A-2, using CRISPR/Cas9 editing


To develop an iPSC SOX9 reporter line for monitoring differentiation into SOX9 expressing cells such as chondrocytes, cranial neural crest and Sertoli cells, we used gene editing to introduce sequences encoding the tdTomato fluorescent protein into the SOX9 locus. The gene-edited line had a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. Endogenous SOX9 expression was undisturbed and the tdTomato fluorescent reporter mirrored SOX9 mRNA expression. This iPSC line will be useful for assessing iPSC differentiation into SOX9-expressing cells and enrichment by cell sorting. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Nur Patria Y, Lilianty J, Elefanty AG, Stanley EG, Labonne T, Bateman JF, Lamandé SR
Journal Stem cell research
Publication Date 2020 Jan;42:101689
PubMed 31884373
DOI 10.1016/j.scr.2019.101689

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