PB001-OI26
MCRIi001-A-1
General
iPSC Genetic Modification
| Disease/phenotype related modifications |
|
Donor Information
General Donor Information |
|
| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA4966491 |
Ethics
Also have a look at the ethics information for the parental line
MCRIi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Integrated DNA Technologies, Addgene |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
iPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
MCRIi001-A.
|
|
Reprogramming method |
|
| Vector type | None |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
None
|
Other |
|
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
iPSC Culture Conditions
| Surface coating | Non coated | |||||||||||||||
| Feeder cells |
MEF |
|||||||||||||||
| Passage method |
Enzymatically
TrypLE
|
|||||||||||||||
| O2 Concentration | 95 % | |||||||||||||||
| CO2 Concentration | 5 % | |||||||||||||||
| Medium |
Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
|||||||||||||||
| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | Yes |
|||||||||||||||
| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | Yes |
|||||||||||||||
| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | Yes |
iPSC Characterisation
Analysis of undifferentiated iPSCs
All analyses published in K. Vlahos, K. Sourris, R. Mayberry, P. McDonald, F.F. Bruveris, J.V. Schiesser, K. Bozaoglu, P.J. Lockhart, E.G. Stanley, A.G. Elefanty, Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults, Stem Cell Res, 34 (2018) 10138.
PMID: 30605840
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (iPSC Line) |
|
| Has the iPSC line karyotype been analysed? |
Yes
Molecular karyotype arr(1-22)x2,(XY)x1
Passage number: 2
Karyotyping method:
Molecular karyotyping by SNP array
|
Other Genotyping (iPSC Line) |
|
Login to share your feedback, experiences or results with the research community.