Generation of NANOS3-mCherry reporter human embryonic stem cell line SYSUe-009-A using CRISPR/Cas9
Summary
NANOS3 is a zinc-finger containing RNA-binding protein that has been demonstrated to be highly expressed in human primordial germ cell(hPGC), thus NANOS3 can serve as a marker for hPGC development. Due to the ethical and technical restrictions, it is difficult to get primary human germline cells. Human primordial germ cell-like cells (hPGCLCs) generated from pluripotent stem cells is an excellent alternatives in human germ cell-related studies. This hESC line with an mCherry knock-in at the site before NANOS3's stop codon serves as a useful tool to learn human PGC specification. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Guo R, Yuan S, Li B, Wang J, Sun C |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Mar;67:103022 |
| PubMed | 36630838 |
| DOI | 10.1016/j.scr.2023.103022 |