Generation of NANOS3-mCherry reporter human embryonic stem cell line SYSUe-009-A using CRISPR/Cas9


NANOS3 is a zinc-finger containing RNA-binding protein that has been demonstrated to be highly expressed in human primordial germ cell(hPGC), thus NANOS3 can serve as a marker for hPGC development. Due to the ethical and technical restrictions, it is difficult to get primary human germline cells. Human primordial germ cell-like cells (hPGCLCs) generated from pluripotent stem cells is an excellent alternatives in human germ cell-related studies. This hESC line with an mCherry knock-in at the site before NANOS3's stop codon serves as a useful tool to learn human PGC specification. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Guo R, Yuan S, Li B, Wang J, Sun C
Journal Stem cell research
Publication Date 2023 Jan 6;67:103022
PubMed 36630838
DOI 10.1016/j.scr.2023.103022

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