Generation and characterization of the human induced pluripotent stem cell line NMIi010-A from peripheral blood mononuclear cells of a healthy 49-year old male individual
Summary
Peripheral-blood derived CD34+ hematopoietic stem and progenitor cells were isolated from a 49-year old male donor and were successfully reprogrammed into human induced pluripotent stem cells (hiPSCs) using integration-free episomal vectors. The hiPSC line exhibited a typical stem cell-like morphology and endogenously expressed several pluripotency markers by concomitant loss of exogenous reprogramming vectors. Genomic integrity was confirmed by microarray-based comparative genomic hybridization (array CGH). Further analysis affirmed the ability of this hiPSC line to differentiate into all three germ layers. Thus, the reported cell line may serve as a healthy control for disease modeling. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Keller AL, Binner A, Breitmeyer R, Vogel S, Anderle N, Rothbauer U, Schenke-Layland K, Schmees C |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Jul;54:102427 |
| PubMed | 34139596 |
| DOI | 10.1016/j.scr.2021.102427 |