General#

Cell Line

hPSCreg Name
NMIi010-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th June 2021
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Provider

Generator NMI Natural and Medical Sciences Institute at the University of Tübingen (NMI)
Distributors

External Databases

BioSamples SAMEA8843280
CLO CLO_0103309
Cellosaurus CVCL_A8MQ

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA8843281

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethik-Kommission an der Medizinischen Fakultät der Eberhard-Karls-Universität und am Universitätsklinikum Tübingen
Approval number 888/2019BO2
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
hematopoietic stem cell
A blood cell progenitor or mother cell representing a slightly later stage than the blast cell; it has the capacity for both replication and differentiation, and has pluripotentiality, giving rise to precursors of various different blood cell lines, such as the proerythrocyte and myeloblast, which cannot self-replicate and must differentiate into more mature daughter cells.
Source cell origin
Peripheral Blood
Blood drawn from a limb.
Synonyms
  • Peripheral Blood

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced
Vector map

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
TRA 1-81
Yes
SSEA-4
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Morphology of a NMIi010-A colony.pdf
Bright field image of a NMIi010-A colony at passage 4 taken with a Zeiss Axiovert microscope
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Morphology
Immunofluorescence staining of the endodermal markers FOXA2 and SOX17_hiPSC line NMIi010-A .pdf
Immunofluorescence staining of the endodermal markers FOXA2 and SOX17_hiPSC line NMIi010-A
Ectoderm
Ont Id: UBERON_0000924
Morphology
Immunofluorescence staining of the ectodermal markers PAX6 and NESTIN_hiPSC line NMIi010-A.pdf
Immunofluorescence staining of the ectodermal markers PAX6 and NESTIN_hiPSC line NMIi010-A

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 18
Karyotyping method: Array CGH

Other Genotyping (Cell Line)