Establishment of Four Induced Pluripotent Stem Cell Lines from CD34+ Hematopoietic Stem and Progenitor Cells from a Patient Diagnosed with an Invasive Lobular Mammary Carcinoma
Summary
CD34+ cells were isolated from peripheral blood of a breast cancer patient. By the introduction of five integration-free episomal vectors, the CD34+ cells were successfully reprogrammed and resulted in four iPSC clones. Flow Cytometry, reverse transcriptase PCR and immunocytochemistry confirm a robust expression of pluripotency factors and the concomitant loss of exogenous reprogramming plasmids. The maintenance of genomic integrity was confirmed by array-based comparative genomic hybridization and iPSCs harbored the capacity to differentiate into all three germ layers. Here, we present the generation and characterization of four iPSC lines that will find application in the field of breast cancer research. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Keller AL, Binner A, Schenke-Layland K, Schmees C |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Oct;64:102902 |
| PubMed | 36055119 |
| DOI | 10.1016/j.scr.2022.102902 |