Generation of a human iPSC line (MPIi006-A) from a patient with Pelizaeus-Merzbacher disease


We established a human induced pluripotent stem cells (hiPSC) line (MPIi006-A) from fibroblasts of a 20-year-old male Pelizaeus-Merzbacher disease (PMD) patient with a hemizygous 643C>T mutation in proteolipid protein 1 (PLP1) gene using a retroviral delivery of OCT4, SOX2, KLF4 and c-MYC. The MPIi006-A iPSC line carried the mutation, displayed typical iPSC morphology, expressed pluripotent stem cell makers, exhibited normal karyotype and were capable of differentiating into cells representative of three germ layers. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Kim KP, Yoon J, Shin B, Röpke A, Han DW, Schöler HR
Journal Stem cell research
Publication Date 2020 Jul;46:101839
PubMed 32446239
DOI 10.1016/j.scr.2020.101839

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