Generation of a human iPSC line (MPIi006-A) from a patient with Pelizaeus-Merzbacher disease
We established a human induced pluripotent stem cells (hiPSC) line (MPIi006-A) from fibroblasts of a 20-year-old male Pelizaeus-Merzbacher disease (PMD) patient with a hemizygous 643C>T mutation in proteolipid protein 1 (PLP1) gene using a retroviral delivery of OCT4, SOX2, KLF4 and c-MYC. The MPIi006-A iPSC line carried the mutation, displayed typical iPSC morphology, expressed pluripotent stem cell makers, exhibited normal karyotype and were capable of differentiating into cells representative of three germ layers. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Kim KP, Yoon J, Shin B, Röpke A, Han DW, Schöler HR|
|Journal||Stem cell research|
|Publication Date||2020 Jul;46:101839|