Generation of a human iPSC line (MPIi006-A) from a patient with Pelizaeus-Merzbacher disease
Summary
We established a human induced pluripotent stem cells (hiPSC) line (MPIi006-A) from fibroblasts of a 20-year-old male Pelizaeus-Merzbacher disease (PMD) patient with a hemizygous 643C>T mutation in proteolipid protein 1 (PLP1) gene using a retroviral delivery of OCT4, SOX2, KLF4 and c-MYC. The MPIi006-A iPSC line carried the mutation, displayed typical iPSC morphology, expressed pluripotent stem cell makers, exhibited normal karyotype and were capable of differentiating into cells representative of three germ layers. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Kim KP, Yoon J, Shin B, Röpke A, Han DW, Schöler HR |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 Jul;46:101839 |
| PubMed | 32446239 |
| DOI | 10.1016/j.scr.2020.101839 |