Generation of two SPAST knockout human induced pluripotent stem cell lines to create a model for Hereditary Spastic Paraplegia type 4


Spastin is a an ATPase that severs microtubules therby regulating amount and mobility of these structures. Mutations in the SPAST gene (SPG4) are the most common form of Hereditary Spastic Paraplegia (HSP). Here, we report the generation of a homozygous and a heterozygous SPAST knockout induced pluripotent stem cell (iPSC) line from a healthy control iPSC line using CRISPR/Cas9 technology. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Korneck M, Wiora L, Schöls L, Hauser S
Journal Stem cell research
Publication Date 2022 Apr;60:102741
PubMed 35248878
DOI 10.1016/j.scr.2022.102741

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