General

Cell Line

hPSCreg Name
ABi002-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 23rd January 2023
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Provider

Generator Moscow Institute of Physics and Technology (National Research University) (AB)
Distributors

External Databases

BioSamples SAMEA112165270

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Ethnicity Caucasion

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Cone dystrophy with supernormal rod response
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Cone dystrophy with supernormal scotopic electroretinogram
  • Cone dystrophy with supernormal rod ERG
  • Cone dystrophy with supernormal rod electroretinogram
Genetic variants
KCNV2 (target)
9p24.2
NM_133497.3: c.754A>T and NM_133497.3: c.775G>A
Heterozygous
Disease associated phenotypes
  • a gradual decrease in visual acuity, photophobia and color perception disorders. Fundus autofluorescence imaging revealed symmetrical disturbances in the fovea of both eyes, Electroretinogram registered a decrease in the amplitude and lengthening of the latency of the b-wave of the maximum in both cone and rod, and a spectral OCT revealed a slight decrease in the average thickness of the neuroepithelium.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
G-band

Donor Relations

All cell lines of this donor's relatives
Has mother:

External Databases (Donor)

BioSamples SAMEA112359103

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: almaqdadalsalloum@gmail.com
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? Yes
Details on restriction to research project
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of Endocrinology Research Center, Moscow, Russia
Approval number 15
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name Peripherla blood mononuclear cells
Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms
Source cell origin
Blood
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
G-banding
Passage number: 13
Karyotyping method: G-Banding

Other Genotyping (Cell Line)