Generation of a fluorescent oligodendrocyte reporter line in human induced pluripotent stem cells
Summary
Human brain organoids can serve as models to study myelination, a process orchestrated by oligodendrocytes. Real-time imaging provides new insights on the communication of oligodendrocytes with neurons as well as demyelination processes in patient derived organoids. PLP1, a prominent myelin protein within the central nervous system, is associated with demyelinating diseases, such as Pelizaeus-Merzbacher. In this study, we generated a stable PLP1-Citrine reporter line (fPLP1) in human induced pluripotent stem cells (iPSCs) by CRISPR/Cas9 editing. fPLP1 facilitates visualization of PLP1 expression in living brain organoids, allowing time-lapse imaging of pre-myelinating and myelinating oligodendrocytes. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Schreiber MK, Zafeiriou MP |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Mar;75:103295 |
| PubMed | 38219302 |
| DOI | 10.1016/j.scr.2023.103295 |