The cell line is not validated yet.

General

Cell Line

hPSCreg Name
MLUi007-J
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UMi042-A-1
(408737 CB9)
Donor diseases:
Alzheimer's Disease
STBCi006-A-1
(ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-1
(iLB-AD + ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-2
(ApoE 2/2)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi012-C
(SFC805-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi013-A
(SFC807-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi013-B
(SFC807-03-04)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-A
(SFC808-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-B
(SFC808-03-04)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
BIOTi001-A
(BIOT-7183-PSEN1)
Donor's gene variants:
PSEN1
Donor diseases:
obsolete_Alzheimer's disease
STBCi014-C
(SFC808-03-06)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi264-A
(SFC142-03-01)
Donor diseases:
Alzheimer disease
STBCi016-A
(SFC810-03-01)
Donor diseases:
Alzheimer disease
STBCi016-B
(SFC810-03-02)
Donor diseases:
Alzheimer disease
STBCi016-C
(SFC810-03-03)
Donor diseases:
Alzheimer disease
UGOTSAi004-A
(N1-003iC3)
Donor diseases:
Alzheimer's Disease
UGOTSAi004-B
(N1-003iC4)
Donor diseases:
Alzheimer's Disease
STBCi032-A
(SFC042-03-01)
Donor diseases:
Alzheimer disease
STBCi032-B
(SFC042-03-03)
Donor diseases:
Alzheimer disease
LBi002-A
(PH-BL-002 Clone 2, iLB-272bfs2)
Donor's gene variants:
CD33, CD33, CD33, CD33
Donor diseases:
Alzheimer disease
LBi002-B
(PH-BL-002 Clone 9, iLB-272bfs9)
Donor's gene variants:
CD33, CD33, CD33, CD33
Donor diseases:
Alzheimer disease
LBi004-A
(PH-BL-204 Clone 210, iLB-278bfs210)
Donor's gene variants:
CD33, CD33, CD33, CD33
Donor diseases:
Alzheimer disease
LBi004-B
(PH-BL-204 Clone 211, iLB-278bfs211)
Donor's gene variants:
CD33, CD33, CD33, CD33
Donor diseases:
Alzheimer disease
STBCi314-A
(BPC335-03-08)
Donor diseases:
Alzheimer disease
SIAISi006-A
(JJY)
Donor diseases:
Alzheimer's Disease
ZZUi010-A
(ZZU-iPS-AD-APP-001)
Donor diseases:
obsolete_Alzheimer's disease
MUNIi021-A
(sAD5)
Donor diseases:
Alzheimer's Disease
ZZUi009-A
(ZZU-iPS-AD-PS1-001)
Donor diseases:
obsolete_Alzheimer's disease
STBCi011-A
(SFC803-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi011-B
(SFC803-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi011-C
(SFC803-03-08)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi012-A
(SFC805-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi256-A
(SFC144-03-02)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi012-B
(SFC805-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
Last update 6th March 2023
Notes This line is part of a set of female iPS cell line obtained from Alzheimer patients and matched controls. The set comprises the following iPS cell lines and APOE genotypes: MLUi007-J (APOE4/4), NLUi008-B (APOE4/3), MLUi009-A (APOE3/3), MLUi010-B (APOE3/3).
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Provider

Generator Medical Faculty of the Martin Luther University Halle-Wittenberg (MLU)

External Databases

BioSamples SAMEA112748717
CLO CLO_0104512
Cellosaurus CVCL_D0IJ

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Ethnicity European

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Alzheimer disease
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic variants
19q13.32
NM_000041.4:c.388T>C
NP_000032.1:p.Cys130Arg
Homozygous
SCV000039738.11
The genotype at base position described by rs7412 is C/C, coding Arg, contributing to ApoE4/E4 variant.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XX
Karyotyping method: mFISH

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA112748718

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Prof. Dr. med. Dan Rujescu, Klinischen Abteilung für Allgemeine Psychiatrie an der Universitätsklinik für Psychiatrie und Psychotherapie (UKPP), Medizinische Universität Wien und AKH Wien
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided:
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website:
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved?
Approval number 275-06
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution

hIPSC Derivation

General

Source cell type
EBV Immortalized Lymphocytes
A biospecimen consisting of lymphocytes that have been isolated from whole blood and are then infected in vitro by Epstein-Barr virus (EBV; HHV4). The infected cells are then cultured to select indefinitely proliferating colonies that can be maintained in tissue culture.
Synonyms
  • EBV Immortalized Lymphocytes

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzymatically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
SSEA-1
No
Alkaline phosphatase staining
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
TBXT
Yes
MEOX1
Yes
PRRX1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
MSI1
Yes
SHH
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: mFISH

Other Genotyping (Cell Line)