The cell line is not validated yet.

General

Cell Line

hPSCreg name MLUi007-J
Cite as:
MLUi007-J (RRID:CVCL_D0IJ)
Cell line type Human induced pluripotent stem cell (hiPSC)
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Last update 3rd January 2024
Notes This line is part of a set of female iPS cell line obtained from Alzheimer patients and matched controls. The set comprises the following iPS cell lines and APOE genotypes: MLUi007-J (APOE4/4), MLUi008-B (APOE4/3), MLUi009-A (APOE3/3), MLUi010-B (APOE3/3).
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Provider

Generator Medical Faculty of the Martin Luther University Halle-Wittenberg (MLU)

External Databases

BioSamples SAMEA112748717
Cellosaurus CVCL_D0IJ
Wikidata Q123033072

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Ethnicity European

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic variants
19q13.32
NM_000041.4:c.388T>C
NP_000032.1:p.Cys130Arg
Homozygous
SCV000039738.11
The genotype at base position described by rs7412 is C/C, coding Arg, contributing to ApoE4/E4 variant.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XX
Karyotyping method: mFISH

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA112748718

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Prof. Dr. med. Dan Rujescu, Klinischen Abteilung für Allgemeine Psychiatrie an der Universitätsklinik für Psychiatrie und Psychotherapie (UKPP), Medizinische Universität Wien und AKH Wien
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Prof. Dr. med. Dan Rujescu, Klinischen Abteilung für Allgemeine Psychiatrie an der Universitätsklinik für Psychiatrie und Psychotherapie (UKPP), Medizinische Universität Wien und AKH Wien
Has the donor agreed to be re-contacted? Unknown

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A biospecimen consisting of lymphocytes that have been isolated from whole blood and are then infected in vitro by Epstein-Barr virus (EBV; HHV4). The infected cells are then cultured to select indefinitely proliferating colonies that can be maintained in tissue culture.
Synonyms
  • EBV Immortalized Lymphocytes

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzymatically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
SSEA-1
No
Alkaline phosphatase staining
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
CXCR4
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
TBXT
Yes
MEOX1
Yes
PRRX1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
MSI1
Yes
SHH
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: mFISH

Other Genotyping (Cell Line)