Generation of six human induced pluripotent stem cell sublines (MZT01E, MZT01F, MZT01N and MZT02D, MZT02G and MZT02H) for reproductive science research


Six human induced pluripotent stem cell sublines (hiPSCs) were generated from human dermal fibroblasts (HDFs) derived from skin biopsies donated from monozygotic twin women wherein one woman had proven fertility and her sister was infertile due to ovarian failure. Three hiPSC sublines were created from each twin's HDFs. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was further verified using PluriTest. We show here that the hiPSC lines created from the twins are equivalent in measures of pluripotency and self-renewal, despite their differential diagnosis. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Pandolfi EC, Sosa E, Hunt TJ, Goldsmith S, Hurlbut K, Silber SJ, Clark AT
Journal Stem cell research
Publication Date 2021 Mar;51:102204
PubMed 33548810
PubMed Central PMC8043044
DOI 10.1016/j.scr.2021.102204

Research Projects

Cell Lines