Pat.2 ARID1B+/- clone 2b (XX)
The cell line is not validated yet.
IMBAi017-A-3
General
Cell Line |
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| hPSCreg name | IMBAi017-A-3 |
| Cite as: | IMBAi017-A-3 (RRID:CVCL_E4GZ) |
| Alternative name(s) |
Pat.2 ARID1B+/- clone 2b (XX)
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 11th December 2024 |
| User feedback | |
Provider |
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| Generator | Institute of Molecular Biotechnology (IMBA) |
External Databases |
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| BioSamples | SAMEA117399235 |
| Cellosaurus | CVCL_E4GZ |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA115296939 |
Ethics
Also have a look at the ethics information for the parental line
IMBAi017-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
IMBAi017-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Medium |
mTeSR™ Plus
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| TRA 1-60 |
Yes |
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| SSEA-4 |
Yes |
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| SSEA5 |
Yes |
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Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
X, X
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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