HVRDe006-A-6

General#

Cell Line

hPSCreg Name HVRDe006-A-6
Cell line type Human embryonic stem cell (hESC)
Last update 20th January 2020
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Provider

Generator Universitätsklinikum Erlangen (UKER)
Owner Universitätsklinikum Erlangen (UKER)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA6621869
Cellosaurus CVCL_YN73

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: Array CGH

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7770418

Ethics#

Also have a look at the ethics information for the parental line HVRDe006-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation#

Date of derivation 2018-07-01
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
one 3,664 kb large deletion ranging from 2q36.3-q37.1 in HVRDe006-A-6
Karyotyping method: Array CGH

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Coffin-Siris Syndrome
Synonyms
  • Coffin-Siris Syndrome
Genetic modifications
ARID1B (target)
Gene knock-out
CRISPR-associated (CRISPR/Cas) System