HVRDe006-A-6
General
Cell Line |
|
| hPSCreg name | HVRDe006-A-6 |
| Cite as: | HVRDe006-A-6 (RRID:CVCL_YN73) |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
HVRDe006-A (HuES6) EHTJUi005-A-1 (ZB11ALD-S16-5) EHTJUi005-A-3 (ZB11ALD-L4) UKEi001-A-1 (CRYABhom) WTSIi227-A-1 (WETU LRP2 KO) IRFMNi003-A-3 (KO PKD1#16) NUIGi038-B-1 (CR.LQTH002Cx-A17) NUIGi038-B-2 (CR.LQTH002Cx-A21) NUIGi038-B-3 (CR.LQTH002Cx-3A15) IRFMNi003-A-4 (KO PKD1#5) NUIGi038-B-4 (CR.LQTH002Cx-3A18) IRFMNi003-A-1 (KO PKD2#17) WAe009-A-62 (KCNQ1 KO) IRFMNi003-A-2 (KO PKD2#36) |
| Last update | 20th January 2020 |
| User feedback | |
Provider |
|
| Generator |
Universitätsklinikum Erlangen (UKER)
Contact:
Universitätsklinikum Erlangen (UKER) |
| Owner | Universitätsklinikum Erlangen (UKER) |
| Distributors | |
| Derivation country | Germany |
External Databases |
|
| BioSamples | SAMEA6621869 |
| Cellosaurus | CVCL_YN73 |
| Wikidata | Q94309941 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
Karyotyping method:
Array CGH
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA7770418 |
Ethics
Also have a look at the ethics information for the parental line
HVRDe006-A
.
| Is there an MTA available for the cell line? | No |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
|
The source cell information can be found in the parental cell line
HVRDe006-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| TRA 1-60 |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
one 3,664 kb large deletion ranging from 2q36.3-q37.1 in HVRDe006-A-6
Karyotyping method:
Array CGH
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
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