CR.LQTH002Cx-3A18
NUIGi038-B-4
General
Cell Line |
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| hPSCreg name | NUIGi038-B-4 |
| Cite as: | NUIGi038-B-4 |
| Alternative name(s) |
CR.LQTH002Cx-3A18
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
NUIGi038-B (LQTH002Cx) NUIGi038-B-1 (CR.LQTH002Cx-A17) NUIGi038-B-2 (CR.LQTH002Cx-A21) NUIGi038-B-3 (CR.LQTH002Cx-3A15) NUIGi038-A (LQTH002C4) NUIGi038-C (LQTH002C1) WAe009-A-1H (MYL3-KO) UKEi001-A-1 (CRYABhom) WAe009-A-36 (JPH2-KO) WAe009-A-1E (TBX20-KO) IRFMNi003-A-3 (KO PKD1#16) IRFMNi003-A-4 (KO PKD1#5) IRFMNi003-A-1 (KO PKD2#17) WAe009-A-62 (KCNQ1 KO) IRFMNi003-A-2 (KO PKD2#36) |
| Last update | 12th August 2021 |
| User feedback | |
Provider |
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| Generator | National University of Ireland Galway (NUIG) |
| Owner | National University of Ireland Galway (NUIG) |
| Derivation country | Ireland |
External Databases |
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| BioSamples | SAMEA9687788 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA5945671 |
Ethics
Also have a look at the ethics information for the parental line
NUIGi038-B
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
NUIGi038-B.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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| Medium |
Essential 8™ Flex
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
CR.LQTH002Cx-3A18 SNP
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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