General

Cell Line
hPSCreg name	UCSDi001-A-4
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	UCSDi001-A-4
Alternative name(s)	UCSD245i-CNTL-1, T036_SNP1_Het_1_iPSC_P15_R01
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	5th April 2025
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Provider
Generator	University of California,San Diego (UCSD) Contact: University of California,San Diego (UCSD)
Owner	University of California,San Diego (UCSD)
Distributors	University of California,San Diego (UCSD)
Derivation country	United States
External Databases
BioSamples	SAMEA117852507
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: allowed Commercial use: allowed
Subclone of	UCSDi001-A

Donor Information

General Donor Information
Sex	female
Ethnicity	EUR
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	Yes Whole genome sequencing https://www.ncbi.nlm.nih.gov/sra/SRX20574733%5baccn Whole genome sequencing was performed on donors’ whole blood as part of the project available at: https://www.ncbi.nlm.nih.gov/bioproject/378454
External Databases (Donor)
BioSamples	SAMEA117851576

Ethics

Also have a look at the ethics information for the parental line UCSDi001-A .

For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line UCSDi001-A.
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Is reprogramming vector detectable?	No
Methods used	PCR, Sequencing
Vector free reprogramming
Other
Derived under xeno-free conditions	Yes
Derived under GMP?	No
Available as clinical grade?	No

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Mechanically
CO2 Concentration	5 %
Medium	mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	Unknown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
SSEA-4	Yes
TRA 1-60	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro directed differentiation

Marker	Expressed
SOX17	Yes
CXCR4	Yes

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro directed differentiation

Marker	Expressed
BRACHYURY	Yes
NCAM	Yes

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro spontaneous differentiation

Marker	Expressed
PAX6	Yes
nestin	Yes

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46,XX; 4.7 Mb chr20q duplication (chr20:29805100-34487478) Karyotyping method: Molecular karyotyping by SNP array http://
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications

Long QT Syndrome 1 Synonyms Romano-Ward Syndrome Long QT Syndrome 1 LQT1 Genetic modifications KCNQ1 (target) Type of modification Isogenic modification Chromosome location 11p15.5 Is the modification homozygous or heterozygous? Homozygous How has the target locus been modified? This cell line is an isogenic control for the UCSDi001-A line. It underwent the same CRISPR protocol but the line does not carry the edited variant

Genetic modifications not related to a disease