KO PKD1#5
IRFMNi003-A-4
General
Cell Line |
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| hPSCreg name | IRFMNi003-A-4 |
| Cite as: | IRFMNi003-A-4 (RRID:CVCL_A9YP) |
| Alternative name(s) |
KO PKD1#5
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
IRFMNi003-A-3 (KO PKD1#16) IRFMNi003-A-1 (KO PKD2#17) IRFMNi003-A-2 (KO PKD2#36) UKEi001-A-1 (CRYABhom) NUIGi038-B-1 (CR.LQTH002Cx-A17) NUIGi038-B-2 (CR.LQTH002Cx-A21) NUIGi038-B-3 (CR.LQTH002Cx-3A15) NUIGi038-B-4 (CR.LQTH002Cx-3A18) WAe009-A-62 (KCNQ1 KO) |
| Last update | 29th November 2019 |
| User feedback | |
Provider |
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| Generator | IRCCS - Istituto di Ricerche Farmacologiche Mario Negri (IRFMN) |
External Databases |
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| BioSamples | SAMEA6453887 |
| Cellosaurus | CVCL_A9YP |
| Wikidata | Q102114248 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA5843913 |
Ethics
Also have a look at the ethics information for the parental line
IRFMNi003-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
IRFMNi003-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
mTeSR™ 1
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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