KO PKD1#5
IRFMNi003-A-4
General
iPSC Line |
|
| hPSCreg name | IRFMNi003-A-4 |
| Cite as: | IRFMNi003-A-4 (RRID:CVCL_A9YP) |
| Alternative name(s) |
KO PKD1#5
|
| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines |
IRFMNi003-A-3 (KO PKD1#16) IRFMNi003-A-1 (KO PKD2#17) IRFMNi003-A-2 (KO PKD2#36) UKEi001-A-1 (CRYABhom) UCSDi001-A-1 (UCSD242i-LQT1-1, LQT1_2_5_iPSC_P17) UCSDi001-A-2 (LQT3_35_iPSC_P15, UCSD243i-LQT3-1) UCSDi001-A-3 (LQT3_4_28_Homo_52_iPSC_P15, UCSD244i-LQT3-2) UCSDi001-A-4 (UCSD245i-CNTL-1, T036_SNP1_Het_1_iPSC_P15_R01) UCSDi001-A-5 (UCSD246i-CNTL-2, T036_SNP1_Het_3_iPSC_P16_R01) NUIGi038-B-1 (CR.LQTH002Cx-A17) NUIGi038-B-2 (CR.LQTH002Cx-A21) NUIGi038-B-3 (CR.LQTH002Cx-3A15) NUIGi038-B-4 (CR.LQTH002Cx-3A18) WAe009-A-62 (KCNQ1 KO) IMBAi001-A-1 (HD.1 ARID1B+/- clone 3b (XX)) |
| Last update | 29th November 2019 |
| User feedback | |
Provider |
|
| Generator | IRCCS - Istituto di Ricerche Farmacologiche Mario Negri (IRFMN) |
External iPSC Databases |
|
| BioSamples | SAMEA6453887 |
| Cellosaurus | CVCL_A9YP |
| Wikidata | Q102114248 |
General iPSC Information |
|
| Publications |
|
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
iPSC Genetic Modification
| Disease/phenotype related modifications |
|
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA5843913 |
Ethics
Also have a look at the ethics information for the parental line
IRFMNi003-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
iPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
IRFMNi003-A.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
iPSC Culture Conditions
| Medium |
mTeSR™ 1
|
iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
|
|||
| NANOG |
Yes |
|
|
|||
| SOX2 |
Yes |
|
||||
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Genotyping
Karyotyping (iPSC Line) |
|
| Has the iPSC line karyotype been analysed? |
Yes
|
Other Genotyping (iPSC Line) |
|
Login to share your feedback, experiences or results with the research community.