HVRDe006-A-1

General

Cell Line

hPSCreg name HVRDe006-A-1
Cite as:
HVRDe006-A-1 (RRID:CVCL_YN14)
Cell line type Human embryonic stem cell (hESC)
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HVRDe006-A
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Last update 10th January 2020
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Provider

Generator Universitätsklinikum Erlangen (UKER)
Owner Universitätsklinikum Erlangen (UKER)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA6613186
Cellosaurus CVCL_YN14
Wikidata Q94309930

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: Array CGH

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7770418

Ethics

Also have a look at the ethics information for the parental line HVRDe006-A .
Is there an MTA available for the cell line? No

hESC Derivation

The source cell information can be found in the parental cell line HVRDe006-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
Self-renewal
Unknown
Endoderm
Unknown
Mesoderm
Unknown
Ectoderm score
Positive
Differentiation Potency

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
normal
Karyotyping method: Array CGH

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Coffin-Siris Syndrome
Genetic modifications
ARID1B (target)
Gene knock-out
CRISPR-associated (CRISPR/Cas) System