CRYABhom
UKEi001-A-1
General
Cell Line |
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| hPSCreg name | UKEi001-A-1 |
| Cite as: | UKEi001-A-1 (RRID:CVCL_C9HF) |
| Alternative name(s) |
CRYABhom
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
UKEi001-A (ERC001sv1162) UKEi001-C (ERC001sv1355) UKEi001-B (ERC001sv1352) IRFMNi003-A-3 (KO PKD1#16) NUIGi038-B-1 (CR.LQTH002Cx-A17) NUIGi038-B-2 (CR.LQTH002Cx-A21) NUIGi038-B-3 (CR.LQTH002Cx-3A15) IRFMNi003-A-4 (KO PKD1#5) NUIGi038-B-4 (CR.LQTH002Cx-3A18) IRFMNi003-A-1 (KO PKD2#17) WAe009-A-62 (KCNQ1 KO) IRFMNi003-A-2 (KO PKD2#36) EHTJUi005-A-1 (ZB11ALD-S16-5) WTSIi227-A-1 (WETU LRP2 KO) |
| Last update | 14th February 2023 |
| User feedback | |
Provider |
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| Generator | University Medical Center Hamburg-Eppendorf (UKE) |
| Owner | University Medical Center Hamburg-Eppendorf (UKE) |
| Distributors | |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA112647719 |
| Cellosaurus | CVCL_C9HF |
| Wikidata | Q123033710 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
| Family history | None |
| Is the medical history available upon request? | On request |
| Is clinical information available? | On request. Healthy heart (MRI, echocardiography). |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
Illumina TruSight Cardio Sequencing Kit.
No pathogenic or likely pathogenic variants detected |
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA6235103 |
Ethics
Also have a look at the ethics information for the parental line
UKEi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | IDT |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
UKEi001-A.
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| Passage number reprogrammed | 29 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
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| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: DMEM/F12: FTDA medium
Main protein source: Albumine Serum concentration: 0 % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-3 |
Yes |
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| NANOG |
Yes |
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SSEA-3: over 90%
Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| Hepatitis B | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XX
Passage number: p32
Karyotyping method:
NanoString nCounter® human karyotype panel
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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| Genetic modifications not related to a disease |
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