NRAP-OE
The cell line is not validated yet.
UKEi001-A-3
General
Cell Line |
|
| hPSCreg name | UKEi001-A-3 |
| Cite as: | UKEi001-A-3 |
| Alternative name(s) |
NRAP-OE
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 4th November 2025 |
| User feedback | |
Provider |
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| Generator | University Medical Center Hamburg-Eppendorf (UKE) |
| Owner | University Medical Center Hamburg-Eppendorf (UKE) |
| Distributors | |
| Derivation country | Germany |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Family history | None |
| Is the medical history available upon request? | On request |
| Is clinical information available? | On request. Healthy heart (MRI, echocardiography). |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
Illumina TruSight Cardio Sequencing Kit.
No pathogenic or likely pathogenic variants detected |
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA6235103 |
Ethics
Also have a look at the ethics information for the parental line
UKEi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | IDT |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
UKEi001-A.
|
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| Passage number reprogrammed | 29 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
Yes |
| Available as clinical grade? |
Yes |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: DMEM/F12:FTDA
Main protein source: Albumine Serum concentration: 0 % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-3 |
Yes |
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| NANOG |
Yes |
Differentiation Potency
In vitro directed differentiation
Protocol or reference
Ecto_Endoderm_induction_RT-QPCR_NRAP-OE.pdf
Endoderm_induction_RT-QPCR_NRAP-OE
In vitro directed differentiation
| Marker | Expressed |
| TNNT2 – troponin T2, cardiac type |
Yes |
Protocol or reference
Mesoderm_induction_FACS_NRAP-OE.pdf
Mesoderm_induction_FACS_NRAP-OE
In vitro directed differentiation
Protocol or reference
Ecto_Endoderm_induction_RT-QPCR_NRAP-OE.pdf
Ectoderm_induction_RT-QPCR_NRAP-OE
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XX
Passage number: 32
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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