TTN_E48_V4623*
BBANTWi012-A-1
General
Cell Line |
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hPSCreg name | BBANTWi012-A-1 |
Cite as: | BBANTWi012-A-1 |
Alternative name(s) |
TTN_E48_V4623*
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | No similar lines found. |
Last update | 10th May 2024 |
User feedback | |
Provider |
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Generator | Biobank Antwerpen (BBANTW) |
Owner | Center of Medical Genetics Antwerp (CMGANT) |
Distributors | |
Derivation country | Belgium |
External Databases |
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BioSamples | SAMEA115499230 |
General Information |
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* Is the cell line readily obtainable for third parties? |
No |
Subclone of |
Donor Information
General Donor Information |
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Sex | female |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
BioSamples | SAMEA115545255 |
Ethics
Also have a look at the ethics information for the parental line
BBANTWi012-A
.
Is there an MTA available for the cell line? | No |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | N.A. |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
BBANTWi012-A.
|
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Vector free reprogramming |
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Other |
|
Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method | Enzymatically |
Medium |
Essential 8™ Flex
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
SOX2 |
Yes |
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TRA 1-81 |
Yes |
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TRA 1-60 |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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Marker | Present | Absent |
mCpG | ||
OCT4 | X |
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
In vitro directed differentiation
Marker | Expressed |
SOX17 |
Yes |
FOXA2 |
Yes |
CXCR4 |
Yes |
In vitro directed differentiation
Marker | Expressed |
HAND1 |
Yes |
NKX2-5 |
Yes |
In vitro directed differentiation
Marker | Expressed |
PAX6 |
Yes |
HES5 |
Yes |
MAP2 |
Yes |
Genotyping
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
Genetic Modification
Disease/phenotype related modifications |
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Genetic modifications not related to a disease |
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