NUIGi038-B-3

CR.LQTH002Cx-3A15

General#

Cell Line

hPSCreg Name
NUIGi038-B-3
Alternative name(s)
CR.LQTH002Cx-3A15
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th August 2021
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Provider

Generator National University of Ireland Galway (NUIG)
Owner National University of Ireland Galway (NUIG)
Derivation country Ireland

External Databases

BioSamples SAMEA9687787
CLO CLO_0103470

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5945671

Ethics#

Also have a look at the ethics information for the parental line NUIGi038-B .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line NUIGi038-B.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium Essential 8™ Flex

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
CR.LQTH002Cx-3A15 SNP
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Long QT Syndrome 3
SCN5A c.1231G>A
Synonyms
  • LQT3
  • Long QT Syndrome 3
Genetic modifications
Isogenic modification
3p22.2
NM_198056:c.1231G>A
NP_932173.1:p.(Val411Met)
Heterozygous
Mutated