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NUIGi038-B-2

Registration Summary:

CR.LQTH002Cx-A21

NUIGi038-B-2

General

Cell Line
hPSCreg name NUIGi038-B-2
Cite as:
NUIGi038-B-2
Alternative name(s)
CR.LQTH002Cx-A21
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Last update 12th August 2021
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Provider
Generator National University of Ireland Galway (NUIG)
Owner National University of Ireland Galway (NUIG)
Derivation country Ireland

External Databases
BioSamples SAMEA9687786

General Information
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information
Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA5945671

Ethics

Also have a look at the ethics information for the parental line NUIGi038-B .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line NUIGi038-B.

Reprogramming method
Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium Essential 8™ Flex

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
CR.LQTH002Cx-A21 SNP
Chromosome(Log2_Ratio)--2032_(CR_LQTH002Cx-A21)_For_cell_line_registration.tif
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Long QT Syndrome 3
SCN5A c.1231G>A
Synonyms
  • LQT3
  • Long QT Syndrome 3
Genetic modifications
Isogenic modification
3p22.2
NM_198056:c.1231G>A
NP_932173.1:p.(Val411Met)
Heterozygous
Mutated