UCSD246i-CNTL-2, T036_SNP1_Het_3_iPSC_P16_R01
The cell line is not validated yet.
UCSDi001-A-5
General
Cell Line |
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| hPSCreg name | UCSDi001-A-5 |
| Cite as: | UCSDi001-A-5 |
| Alternative name(s) |
UCSD246i-CNTL-2, T036_SNP1_Het_3_iPSC_P16_R01
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 24th March 2025 |
| User feedback | |
Provider |
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| Generator | University of California,San Diego (UCSD) |
| Owner | University of California,San Diego (UCSD) |
| Distributors | |
| Derivation country | United States |
External Databases |
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| BioSamples | SAMEA117852522 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | EUR |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
Whole genome sequencing, RNA-Seq, ATAC-seq
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External Databases (Donor) |
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| BioSamples | SAMEA117851576 |
Ethics
Also have a look at the ethics information for the parental line
UCSDi001-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
UCSDi001-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR, Sequencing
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Mechanically |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Unknown |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
No characterisation data could be found for this subclone. Please open parental cell line UCSDi001-A .Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
4.7 Mb chr20q duplication (chr20:29805100-34487478)
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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