UCSD182i-3-2, iPSCORE_3_2
UCSDi001-A
General
Cell Line |
|
| hPSCreg name | UCSDi001-A |
| Cite as: | UCSDi001-A (RRID:CVCL_EP25) |
| Alternative name(s) |
UCSD182i-3-2, iPSCORE_3_2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 4th April 2025 |
| User feedback | |
Provider |
|
| Generator | University of California,San Diego (UCSD) |
| Owner | University of California,San Diego (UCSD) |
| Distributors | |
| Derivation country | United States |
External Databases |
|
| Cellosaurus | CVCL_EP25 |
| Wikidata | Q54989943 |
| BioSamples | SAMEA117852148 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
| Subclones | |
Donor Information
General Donor Information |
|
| Sex | female |
| Age of donor (at collection) | 25-29 |
| Ethnicity | EUR |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Yes
46,XX; 4.7 Mb chr20q duplication (chr20:29805100-34487478)
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Whole genome sequencing
https://www.ncbi.nlm.nih.gov/sra/SRX20574733%5baccn Whole genome sequencing was performed on donors’ whole blood as part of the project available at: https://www.ncbi.nlm.nih.gov/bioproject/378454 |
External Databases (Donor) |
|
| BioSamples | SAMEA117851576 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| a public organisation? | Yes |
| a non-profit company? | Yes |
| a for-profit corporation? | Yes |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | RB committee at the University of California San Diego and The Salk Institute |
| Approval number | IRB # 110776ZF |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
hIPSC Derivation
General |
|
| Source cell type |
Any skin fibroblast that is part of some dermis.
|
| Source cell origin |
An elbow contracture that limits the ability of the elbow joint to be extended (straightened), meaning that the elbow is fixed in an flexed (bent) position.
Synonyms
|
| Age of donor (at collection) | 25-29 |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR, Sequencing
|
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Mechanically |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Pluripotency Score | Novelty Score | Link to microarray data |
| 21,15117348 | 1,397952435 | https://doi.org/10.1016/j.stemcr.2017.03.012 |
Transcriptome Characterisation
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
In vitro directed differentiation
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46,XX; 4.7 Mb chr20q duplication (chr20:29805100-34487478)
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
RNA-seq, ATAC-seq
https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs000924.v5.p2&phv=292604&phd=7225&pha=&pht=6323&phvf=&phdf=&phaf=&phtf=&dssp=1&consent=&temp=1 Other genomic data, such as RNA-seq and ATAC-seq,have been generated from the iPSC line |
Login to share your feedback, experiences or results with the research community.