ERC001sv1355

General

Cell Line

hPSCreg name UKEi001-C
Cite as:
UKEi001-C (RRID:CVCL_C9HG)
Alternative name(s)
ERC001sv1355
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKEi001-A
(ERC001sv1162)
UKEi001-B
(ERC001sv1352)
UKEi001-A-1
(CRYABhom)
Last update 12th January 2023
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator University Medical Center Hamburg-Eppendorf (UKE)
Owner University Medical Center Hamburg-Eppendorf (UKE)
Derivation country Germany

External Databases

BioSamples SAMEA112286109
Cellosaurus CVCL_C9HG
Wikidata Q123033711

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 60-64

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history None
Is the medical history available upon request? On request
Is clinical information available? On request. Healthy heart (MRI, echocardiography).

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Illumina TruSight Cardio Sequencing Kit.
No pathogenic or likely pathogenic variants detected

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6235103

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? Yes
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? Yes
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: Double pseudomized
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethik-Kommision der Ärztekammer Hamburg
Approval number PV4798/28.10.2014
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethik-Kommision der Ärztekammer Hamburg
Approval number PV4798/28.10.2014
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? Yes
Further constraints on use Material will be anonymised after 10 years
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.
Source cell origin
The underside concavity where the arm and the shoulder are joined.
Synonyms
  • Axilla
  • AXILLA
  • Armpit
  • axilla
  • Underarm
  • arm pit
  • armpit
  • axillary region
  • regio axillaris
show more synonyms
Source cell type (free text) http://purl.obolibrary.org/obo/NCIT_C12674
Age of donor (at collection) 60-64
Collected in 2015

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones Morphology, growth rate
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F12: FTDA medium
Main protein source: Albumine
Serum concentration: 0 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Lin28
Yes
NANOG
Yes
SSEA-3
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Pax 6
Yes

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 23
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Nanostring